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Abcam’s anti-Herpes simplex virus Type 1 (HSV1) IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Herpes simplex virus Type 1 in Human serum and plasma.
A 96-well plate has been precoated with Herpes simplex virus Type 1 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Herpes simplex virus Type 1-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Herpes simplex virus Type 1 IgM sample captured in plate.
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