ELABSCIENCE Rat SOD1 (Superoxide Dismutase 1 Soluble) ELISA KIT


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Rp. 9,309,970
Katalog E-EL-R1424
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Brand Elabscience
Kondisi New
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Rat SOD1 (Superoxide Dismutase 1 Soluble) ELISA KIT  Elabscience

Principle :

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with SOD1. During the reaction, Rat SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat SOD1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat SOD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Description :

Full Name   Rat SOD1( Superoxide Dismutase 1, Soluble) ELISA Kit
  Product ID   E-EL-R1424   Organism   Rat
  Package   96T   Price   Inquire
  Detection Range   0.156~10ng/mL   Sensitivity   0.094ng/mL

Components :

Item   Specifications   Storage
  Micro ELISA Plate   8 wells ×12 strips   4?
  Reference Standard   2 vials   4?
  Reference Standard & Sample Diluent   1vial 20mL   4?
  Concentrated Biotinylated Detection Ab   1vial 120μL   4?
  Biotinytated Detection Ab Diluent   1vial 10mL   4?
  Concentrated HRP Conjugate   1vial 120μL   4?(shading light)
  HRP Conjugate Diluent   1vial 10mL   4?
  Concentrated Wash Buffer (25×)   1vial 30mL   4?
  Substrate Reagent   1vial 10mL   4?(shading light)
  Stop Solution   1vial 10mL   4?
  Plate Sealer   5 pieces  
  Manual   1 copy  
  Desiccant   1 pack  
  Certificate of Analysis   1 copy

 

Procedure :

1. Add 50μL standard or sample to each well.

2. Immediately add 50μL Biotinylated Detection Ab to each well.

3. Incubate for 45 minutes at 37?.

4. Aspirate and wash 3 times.

5. Add 100μL HRP Conjugate to each well. Incubate for 30 minutes at 37?.

6. Aspirate and wash 5 times.

7. Add 90μL Substrate Reagent. Incubate 15 minutes at 37?.

8. Add 50μL Stop Solution. Read at 450nm immediately.

9. Calculation of results.

5

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