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Rat SOD1 (Superoxide Dismutase 1 Soluble) ELISA KIT Elabscience
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with SOD1. During the reaction, Rat SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat SOD1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat SOD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
|Full Name||Rat SOD1( Superoxide Dismutase 1, Soluble) ELISA Kit|
|Micro ELISA Plate||8 wells ×12 strips||4?|
|Reference Standard||2 vials||4?|
|Reference Standard & Sample Diluent||1vial 20mL||4?|
|Concentrated Biotinylated Detection Ab||1vial 120μL||4?|
|Biotinytated Detection Ab Diluent||1vial 10mL||4?|
|Concentrated HRP Conjugate||1vial 120μL||4?(shading light)|
|HRP Conjugate Diluent||1vial 10mL||4?|
|Concentrated Wash Buffer (25×)||1vial 30mL||4?|
|Substrate Reagent||1vial 10mL||4?(shading light)|
|Stop Solution||1vial 10mL||4?|
|Plate Sealer||5 pieces|
|Certificate of Analysis||1 copy|
1. Add 50μL standard or sample to each well.
2. Immediately add 50μL Biotinylated Detection Ab to each well.
3. Incubate for 45 minutes at 37?.
4. Aspirate and wash 3 times.
5. Add 100μL HRP Conjugate to each well. Incubate for 30 minutes at 37?.
6. Aspirate and wash 5 times.
7. Add 90μL Substrate Reagent. Incubate 15 minutes at 37?.
8. Add 50μL Stop Solution. Read at 450nm immediately.
9. Calculation of results.
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